WebNov 1, 1976 · The Eadie-Hofstee Scatchard plot is rather unusual in that all of the graph can be seen in one compass, i.e. there are no linear asymptotes as in all the other … Webligand interactions (Scatchard plots) and of the kinetics of enzymatic reactions (plots according to the Michaelis-Menten equation and its various linearized forms). Theoretical For a simple acid-base equilibrium ... equivalent to an Eadie plot. 3 …

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Web'Eadie Scatchard Plot Physics Forums November 24th, 2011 - So It Is Particularly Easy To Plot The Results In μM Units The Slope Is 1 K S Or If You Rotate The Graph Use The Axes The Other Way Round The Slope Is K S This Gives A K S In The Region Of 3μM Just Plot The Results S Bound Against S Free WebMar 18, 2016 · Segel's book also has an "Eadie-Scatchard" plot (no Hofstee; p. 215, Fig. IV-23), which is exactly identical, except the axes are switched: v/S on the Y axis and v … cookstown dog boarding

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WebNov 28, 2024 · 1 Answer. The Scatchard eqn can be written as Y [ L] = n K − Y K with [ L] the free ligand concentration and Y the fraction bound, (i.e. [ L] bound /total protein) so the x-axis intercept (when Y / [ L] = 0) is n the number of ligands bound, the slope is − 1 / K and the y-axis intercept is n / K. Your plot confirms this equation, the ... WebApr 10, 2024 · The Scatchard plot is better than the Eadie–Hoffstee plot for binding data because it depends more on the values at high ligand concentration, the most … WebJan 1, 1982 · The Eadie-Scatchard plot, which is the most sensitive graphical technique for detecting deviations from Michaelis-Menten kinetics, was used. In the case of phosphatases extracted from two soils and of the benzoylarginineamide hydrolysing protease extracted from one soil the relationship between V / S and V produced two straight lines. cookstown gymnastics