Fitc-conjugated pac-1

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August undefined, 2024

WebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs. WebAug 2, 2010 · The molar ratio of FITC and polymer taken was 1:20. The labeled polymer solution was dialysed against PBS (pH 7.4) for 24 h and then distilled water for 24 h (MWCO 500 for PEI 600, MWCO 1000 for PEI 1800, MWCO 3500 for PEI 25k) until free FITC could not be detected by TLC (chloroform/methanol = 1/1, v/v). ... After conjugation with TA, …

IHC-plus™ ADCYAP1R1 / PAC1 Receptor Polyclonal Antibody …

WebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, … WebClone HIT3a. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD3 (Cat. No. 561802/555539; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. … citrasolvedeep discount

(340507) BD™ FITC Mouse Anti-Human PAC-1 - CiteAb

WebConjugate: FITC. Order Details. View Available Conjugates. Catalog# & Conjugate Size Price; Alexa Fluor 350 NBP2-62201AF350: Alexa Fluor 405 NBP2-62201AF405 ... (PAC-1) [FITC] and receive a gift card or discount. Submit a Review. Bioinformatics. Gene Symbol: ITGA2B Popular Products ... WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. WebDec 1, 2024 · The mono-conjugated FITC-insulin (22%) with unlabeled insulin prepared from 0.25:1 molar ratio (Fig. 3 A), tri-conjugated FITC-insulin (84%) with little amount of di-conjugate prepared from 4:1 molar ratio (Fig. 2 D) and the unlabeled insulin were chosen for the transport study. The percentage amount of mono-conjugate and tri-conjugate … dickinson county arrest log

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Anti Pac1 Fitc Becton Dickinson Bioz

WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. WebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. citra solv hobby lobbyWebIHC-plus™ ADCYAP1R1 / PAC1 Receptor Antibody LS-A109382 is a pathologist validated IHC antibody FITC Rabbit anti-Human ADCYAP1R1 / PAC1 Receptor. Validated for IHC. Tested on 20 paraffin-embedded human tissues. ... Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.) Purification. citra-solv household and automotive cleaner

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Fitc-conjugated pac-1

Weball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... WebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on …

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WebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor. WebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and …

WebIn Fig. 1a, we used FITC- conjugated anti-human fibrinogen antibody to highlight intercellular reactivity (white dots, red arrow). The round structures (blue arrow) seem to be a series of TTS ... Web(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 …

WebAnti Pac1 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results ... 1) Product Images from "Red blood cell-derived phosphatidylserine positive extracellular vesicles are associated with past ... WebThe PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a IIb ß 3. PAC-1 binding to fibrinogen is pH and Ca 2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher.

WebFITC Protocols Fluorescein Isothiocyanate (FITC) - conjugated Antibodies GFP Protocols › Immunoprecipitation Protocols › Membrane Protein Protocols › Protein Expression Protocols › Protein Isolation Protocols › Protein Purification Protocols › SDS PAGE Protocols ›

WebFITC Conjugated Anti-PAC-1 Monoclonal Antibody (Clone:PAC-1) Share: Figure 1: Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC. citra solve where to buyWebsupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1 citra satelit google earthWebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. citra solv msds sheetWebSpecifications. Name. Anti-PAC-1 Antibody [PAC-1] (FITC) Description. Mouse monoclonal [PAC-1] antibody to PAC-1 (FITC). Specificity. This antibody recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation ... dickinson county attorney kansasWebUse RGDS with conjugated PAC-1 antibody NOTE: Some commercial preparations can include lipopolysaccharide (LPS), a potent platelet activator. FACS brand flow cytometer. Refer to the appropriate instrument user’s guide for detailed information. ... stain with PAC-1 FITC/CD62P PE/CD61 PerCP in the presence of RGDS to inhibit PAC-1 binding ... dickinson county animal shelter michiganWebData on immunogenicity of adenovirus-vectored vaccine in chronic obstructive pulmonary disease (COPD) patients is limited. Therefore, we aimed to determine the humoral and cellular immune responses after homologous ChAdOx-1 vaccination in subjects with COPD. COPD subjects and age- and sex-matched healthy elderly receiving ChAdOx-1 … citrasolv lowesWebSep 18, 2024 · FITC-conjugated anti-human CD62P (P-selectin) and FITC-conjugated anti-human PAC-1 were purchased from BioLegend (California, USA). Fluo-3 AM calcium indicators and lactate dehydrogenase (LDH) … dickinson county atv trail map