Phosphinothricin resistance
WebNov 1, 1996 · Wild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to the ammonium salt of l-phosphinothricin (PPT, also called glufosinate), an irreversible inhibitor of glutamine... WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine R. J ARDAK -J AMOUSSI 1) , B. B OUAMAMA 1) , A. M LIKI 1) , A. G HORBEL 1) and G. M. R EUSTLE 2)
Phosphinothricin resistance
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WebBialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops1–4. Webresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the
WebAug 21, 2024 · To investigate the effect of the endogenous gpd intron 1 on protein expression in G. lucidum, we constructed plasmids in which the heterologous codon-optimized phosphinothricin-resistance gene (opbar)-flag was regulated by the endogenous gpd promoter and the succinate dehydrogenase gene (sdh) terminator of G. lucidum (pJW … WebPhosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper).
WebResistance plasmids include pGreen II 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos- bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ … Web2 14 Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) 15 genes, which convey resistance to the broad-spectrum herbicide phosphinothricin 16 (also known as glufosinate) via N-acetylation, have been globally used in basic plant 17 research and genetically engineered crops1-4.Although early in vitro enzyme assays 18 showed that …
WebPhosphinothricin. Resistance against PTT can be achieved by elevated levels of GS, which protect bacteria from the action of PTT: the increased activity of GSII in S. …
WebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to PPT, but it was demonstrated that resistance is due to lack of PPT transport into the cells, which strongly suggests that PPT and l-methionine-S-sulfoximine enter the cells through different systems. Wild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant … small pond in a potWebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the … small pond fountains with lightsWebApr 11, 2024 · insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate … highlights hair short hairWebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the assimilation of ammonia accumulated as a result of photorespiration and nitrate reduction. small pond in the woodsGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and … See more In the 1960s and early 1970s, scientists at University of Tübingen and at the Meiji Seika Kaisha Company independently discovered that species of Streptomyces bacteria produce a tripeptide they called bialaphos that … See more Phosphinothricin is a glutamine synthetase inhibitor that binds to the glutamate site. Glufosinate-treated plants die due to a buildup of ammonia in the thylakoid lumen, leading to the uncoupling of photophosphorylation. The uncoupling of photophosphorylation … See more Glufosinate is a United States Environmental Protection Agency EPA registered chemical. It is also a California registered chemical. … See more Glufosinate is a broad-spectrum herbicide that is used to control important weeds such as morning glories, hemp sesbania (Sesbania bispinosa), Pennsylvania smartweed ( See more Exposure to humans in foods As glufosinate is often used as a pre-harvest desiccant, residues can also be found in foods that humans ingest. Such foods include … See more • BASF's site of LibertyLink crops • "Basta technical guide" (PDF). Archived from the original (PDF) on 2024-09-02. • Glufosinate in the Pesticide Properties DataBase (PPDB) See more highlights hangoutWebNov 27, 2024 · Phosphinothricin resistance is a widely used trait in plant science research and agriculture. The BAR resistance gene that inactivates phosphinothricin has now been … small pond for gardenWebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl-(methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.PPT is a structural analog of glutamate that binds to glutamine … highlights hairstyles for women