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Phospho-mtor antibody

Web80596-1-RR targets Phospho-mTOR (Ser2448) in WB, ELISA applications and shows reactivity with Human, Rat samples. Background Information MTOR, also named as … WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ...

Phospho-mTOR (Ser2448) Polyclonal Antibody - Thermo …

Webtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ... WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … grabber drywall cart https://northernrag.com

Anti-phospho-mTOR (Thr2446) Antibody - Sigma-Aldrich

WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 ... Webadult animals (c) Immunoprecipitation (IP) with mTOR antibody efficiently extracts mTOR complexes from prepared lysates. mTOR is highly enriched in the IP fraction but is absent … WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold grabber drywall screws submittal sheets

p-mTOR Antibody (59.Ser 2448) SCBT - Santa Cruz …

Category:torc-specific phosphorylation of mtor phospho-ser2481 does

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Phospho-mtor antibody

Anti-mTOR (phospho S2448) antibody (ab131538) Abcam

WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl … WebPhospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site …

Phospho-mtor antibody

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WebCatalogue Number. ABS88. Description. Anti-phospho-mTOR (Thr2164) Antibody. Alternate Names. Serine/threonine-protein kinase mTOR. FK506-binding protein 12-rapamycin … WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody.

Webindicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. mTOR Antibodies Phospho-Specific mTOR Antibodies mTOR CRISPR Knockout, HDR and Nickase Knockout Plasmids mTOR CRISPR Activation Plasmids mTOR siRNA, shRNA Plasmid and shRNA Lentiviral Particles gene silencers WebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide …

WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein.

WebDescription: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. …

WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … grabber fall protectionWebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot. - Find MSDS or SDS, a COA, data sheets and more information. grabber durock screwsWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. grabber exterior wood screwWebPolyclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1872 publications. Validated for Western Blotting, Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-mTOR (Ser2448) Antibody (CST #2971) is ready to … grabber drywall to drywall screwsWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... grabber fastener companyWebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for … grabber examplesWebtorc-specific phosphorylation of mtor phospho-ser2481 does is a marker for intact mtorc2.pdf. 2016-06-15 ... grabber fine thread screw submittal