WebFeb 13, 2015 · Immunoblot data showed that the phosphopeptide but not the peptide without the phosphoserine eluted the AKB1 from the co-IP complexes efficiently, ... About 5 µl of each eluates were subjected to WB using anti-AKB1 Ab (left panel) and kinase assay using PKC Pep-Tag assay kit substrate (right panel). ... WebPhosphoserine is a product of glycolysis and is formed by amino group transfer from glutamic acid to phosphohydroxypyruvic acid. Low levels of phosphoserine may be …

Phosphoserine Antibody (NB100-1953): Novus Biologicals

WebJan 2, 2007 · In the case of phosphoserine Ab, blots were blocked for 1 h at room temperature, probed (1:1000) overnight at 4 °C and incubated with HRP-conjugated goat anti-rabbit Ab (1:2000) for 1 h at room temperature. Signal was developed using enhanced chemiluminescence Western blot detection reagents (Pierce).3. Results3.1. literature and medicine journal

Why twenty amino acid residue types suffice(d) to support all …

WebPhosphoserine Antibodies. Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits … WebBecause MEKK1 contains multiple serine phosphoracceptor residues we used an anti-phosphoserine Ab to analyze the kinetics of their activation in T cells (Fig. 3 A ). MEKK1 becomes activated with... WebThe anti-phosphoserine and anti-phosphothreonine Abs are from Millipore, Cat# AB1603 & AB1607 respectively. Protocol 2 - Immunoprecipitation using Phosphotyrosine Antibody Reagents Use as indicated at 5 µl per IP reaction (200-500 µg total lysate per IP), sufficient for approximately 40 IP assays. Method 1. Add 5 µl of anti-phosphotyrosine (Cat. literature and music