Webb25 dec. 2001 · Domain IV of mouse perlecan, which consists of 14 immunoglobulin superfamily (IG) modules, was prepared from recombinant human cell culture medium in the form of two fragments, IV-1 (IG2–9, 100 kDa) and IV-2 (IG10–15, 66 kDa). Webb25 dec. 2001 · Recombinant Drosophila perlecan domain V could be obtained in similar yields from mammalian cells (3–4 μg·mL −1) as described previously for mouse perlecan domain V . The purified product shifted from a 100-kDa band to a 90-kDa band when nonreduced, indicating the formation of internal disulfide bridges ( Fig. 2B ).
Recombinant Domain V of Human Perlecan Is a Bioactive Vascular …
Webb28 aug. 2024 · In particular, C-terminal domain V of perlecan expressed as a proteoglycan decorated with either a heparan sulfate (HS) or a chondroitin sulfate (CS) GAG chain (rDV) is particularly promising in ... Webb28 aug. 2024 · Recombinant forms of domain V have been proposed as therapeutic agents for the treatment of cancer, stroke, and the development of cardiovascular devices and … galway bottomless brunch
Perlecan domain V of Drosophila melanogaster - Friedrich - 2000 ...
Webb28 aug. 2024 · Perlecan domain V promotes tissue repair through interactions with VEGF, VEGF-R2 and α2β1 integrin. Perlecan domain-V LG1-LG2 and LG3 fragments antagonize … Webb25 dec. 2001 · Perlecan domain V is the only recombinant fragment that has previously been examined for nidogen-1 binding and showed a K d of 56–78 n m . We therefore … WebbRecombinant forms of domain V have been proposed as therapeutic agents for the treatment of cancer, stroke, and the development of cardiovascular devices and … black country mineral safeguarding area